Human Cd209 Antigen, CD209 ELISA Kit

Dendritic cell (DC) -specific intercellular adhesion molecule 3 (ICAM-3) grabbing nonintegrin (DC-SIGN), also known as CD209, is a type II transmembrane protein on DCs with a C-type lectin extracellular domain, is capable of binding ICAM-3 on resting T cells in the secondary lymphoid organs, providing the initial contact between these cells during the establishment of cell-mediated immunity. It is not only a pattern recognition receptor but implicated in immunoregulation of DCs. It has an important role in mediating DC adhesion, migration, inflammation, activating primary T cell, triggering immune response and participating in immune escape of pathogens and tumors. DC-SIGN also mediates the capture and internalization of viral, bacterial, and fungal pathogens by dendritic cells, such as HIV-1, Ebola virus, cytomegalovirus, Dengue virus, and hepatitis C virus. DC-SIGN is unique in that it regulates adhesion processes, such as DC trafficking and T-cell synapse formation, as well as antigen capture. Moreover, even though several C-type lectins have been shown to bind HIV-1, DC-SIGN does not only capture HIV-1 but also protects it in early endosomes allowing HIV-1 transport by DC to lymphoid tissues, where it enhances trans infection of T cells. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 1500 pg/ml | 2.544 | 2.426 | 2.295 2.309 750 pg/ml | 1.314 | 1.307 | 1.176 1.301 375 pg/ml | 0.692 | 0.699 | 0.568 0.707 187.5 pg/ml | 0.39 | 0.392 | 0.261 0.395 93.75 pg/ml | 0.242 | 0.247 | 0.116 0.253 46.875 pg/ml | 0.172 | 0.166 | 0.035 0.161 23.4375 pg/ml | 0.14 | 0.136 | 0.005 0.133 0 pg/ml | 0.13 | 0.131 | 0 0.133

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 94.6 | 6.8 | 7.2 Intra-assay | 2 | 20 | 473 | 29.7 | 6.3 Intra-assay | 3 | 20 | 1051 | 66 | 6.3 Inter-assay | 1 | 20 | 95 | 6.1 | 6.4 Inter-assay | 2 | 20 | 474 | 25.6 | 5.4 Inter-assay | 3 | 20 | 1049 | 69.9 | 6.7

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail