Pan Cytokeratin Antibody Cocktail (Acidic + Basic)
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67kDa (CK1) ; 64kDa (CK3) ; 59kDa (CK4) ; 58kDa (CK5) ; 56kDa (CK6) ; 52kDa (CK8) ; 56.5kDa (CK10) ; 50kDa (CK14) ; 50kDa (CK15) ; 48kDa (CK16) ; 40kDa (CK19) . Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
Q7Z794; Q01546
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Human epidermal keratin was used as the immunogen for the Pan Cytokeratin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPM115 + SPM116
Applications
FACS, IF, WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Pan Cytokeratin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Pan Cytokeratin antibody is available for research use only.
Storage Conditions
Aliquot the Pan Cytokeratin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Pan Cytokeratin antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Frequently Asked Questions
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