ENAH Antibody / MENA

The Wiskott-Aldrich syndrome (WAS) is characterized by thrombocytopenia, eczema, defects in cell-mediated and humoral immunity and a propensity for lymphoproliferative diseases. The syndrome is the result of a mutation in the gene encoding a proline-rich protein termed WASP. WASP is a downstream effector of Cdc42 and has been implicated in actin polymerization and cyto- skeletal organization. Distantly related proteins, VASP (vasodilator-stimulated phosphoprotein) and Mena (for mammalian enabled protein), are involved in the regulation of cytoskeletal dynamics. Both Mena and VASP accumulate at focal adhesions. Mena is highly expressed in the developing nervous system and may be involved in growth cone motility and axon guidance.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA/sodium azide-free format for coating), Western blot: 1-2 µg/mL

UniProt

Q8N8S7

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 485-589 from the human protein was used as the immunogen for the ENAH antibody.

Clonality

Monoclonal

Isotype

IgG2c κ

Clone

ENAH/1988

Applications

ELISA, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the ENAH antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This ENAH antibody is available for research use only.

Storage Conditions

Store the ENAH antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the ENAH antibody may be required due to differences in protocols and secondary/substrate sensitivity.