PKD2 (phospho Ser876) Polyclonal Antibody

Rabbit Anti-PKD2 (phospho Ser876) Polyclonal Antibody

Product Specifications

Background

Protein kinase D2 encoded by PRKD2 belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. Protein kinase D2 can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms.

CAS Number

9007-83-4

Product Name Alternative

PRKD2; PKD2; HSPC187; Serine/threonine-protein kinase D2; nPKC-D2

Gene ID

25865

Accession Number

Q9BZL6

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human PKD2 around the phosphorylation site of S876

Clonality

Polyclonal

Tag

Serine threonine-protein kinase D2

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

96kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-PKD2 (S876) Polyclonal Antibody detects endogenous levels of PKD2 protein only when phosphorylated at S876.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200