Rabbit anti-PDE1B Antibody
Product Specifications
Background
Rabbit polyclonal antibody to PDE1B
CAS Number
9007-83-4
Synonyms
PDE1B1; PDES1B; Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B; Cam-PDE 1B; 63 kDa Cam-PDE
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human PDE1B
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of PDE1B expression in THP1 (A), mouse brain (B), mouse spleen (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of PDE1B staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
5153
Gene ID (Mouse)
18574
Gene ID (Rat)
29691
SwissProt (Human)
Q01064
SwissProt (Mouse)
Q01065
SwissProt (Rat)
Q01066
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