Rabbit anti-DDAH2 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to DDAH2
CAS Number
9007-83-4
Synonyms
DDAH; G6A; NG30; N (G), N (G) -dimethylarginine dimethylaminohydrolase 2; DDAH-2; Dimethylarginine dimethylaminohydrolase 2; DDAHII; Dimethylargininase-2; Protein G6a; S-phase protein
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human DDAH2
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of DDAH2 expression in SW620 (A), MCF7 (B), mouse lung (C), mouse kidney (D), rat heart (E), rat brain (F) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of DDAH2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
23564
Gene ID (Mouse)
51793
Gene ID (Rat)
294239
SwissProt (Human)
O95865
SwissProt (Mouse)
Q99LD8
SwissProt (Rat)
Q6MG60
Available Sizes
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