Rabbit anti-DDAH2 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to DDAH2

CAS Number

9007-83-4

Synonyms

DDAH; G6A; NG30; N (G), N (G) -dimethylarginine dimethylaminohydrolase 2; DDAH-2; Dimethylarginine dimethylaminohydrolase 2; DDAHII; Dimethylargininase-2; Protein G6a; S-phase protein

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human DDAH2

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of DDAH2 expression in SW620 (A), MCF7 (B), mouse lung (C), mouse kidney (D), rat heart (E), rat brain (F) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of DDAH2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.