RIPK2 Kinase Assay Kit

The RIPK2 Kinase Assay Kit is designed to measure RIPK2 kinase activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.MBP (Myelin Basic Protein) is a non-specific protein substrate that is used as a "universal substrate" for many in-vitro kinase activity assays. This protein is targeted by many serine/threonine kinases at conserved amino acids. We use the dephosphorylated version of the MBP substrate in our assays to determine the kinase-mediated phosphorylation of MBP. Our assays are not suitable for studying autophosphorylation of the kinase due to the presence of the MBP substrate.Need us to run inhibitor screens or profile your compounds against RIPK2? Check out ourKinase Screening Services.

Product Specifications

Background

RIPK2 (receptor-interacting protein kinase 2) has been shown to mediate the activation of pro-inflammatory signals by NODs (Nucleotide-binding oligomerization domain-containing proteins), suggesting RIPK2 may be a promising therapeutic target in autoimmune and inflammatory diseases.

UniProt

O43353

Format

Catalog #ComponentAmountStorage40173RIPK25 µg-80°CAvoid multiple freeze/ thaw cycles!793345x Kinase assay buffer1.5 ml-20°C79686ATP (500 µM) 100 µl-20°C78514MBP (5 mg/ml) 100 µl-20°C7969696-well plate, white1Room Temp