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ERK1/2 Antibody / MAPK1/3

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000

UniProt

P27361, P28482

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a portion of human ERK1 was used as the immunogen for this ERK1/2 antibody.

Clonality

Monoclonal

Isotype

LgG1

Clone

4F3-A10-E7

Applications

WB

Purity

Protein G affinity

Format

Purified

Buffer

Antibody in PBS with 50% Glycerol and 0.03% ProClin 300

Reconstitution

Store the ERK1/2 antibody at -20oC.

Limitations

This ERK1/2 antibody is available for research use only.

Storage Conditions

Store the ERK1/2 antibody at -20°C.

Formulation

In PBS with 50% glycerol and 0.03% ProClin 300

Applications Notes

The stated application concentrations are suggested starting points. Titration of the ERK1/2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of human Jurkat, human A549, rat C6, moouse NIH3T3 and human HeLa cell lysates using ERK1/2 antibody at 1:1000. Predicted molecular weight: ~44 kDa (ERK1), ~42 kDa (ERK2) .

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