NSE Antibody

Neuron Specific Enolase, also called Gamma-enolase, and Enolase 2 (ENO2) has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity) . [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

UniProt

P09104

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 6-32 from the human protein was used as the immunogen for this NSE antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the NSE antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This NSE antibody is available for research use only.

Storage Conditions

Aliquot the NSE antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the NSE antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Rat

Image Legend

Western blot analysis of NSE antibody and (1) mouse cerebellum tissue and (2) Y79, (3) HepG2, (4) Jurkat and (5) CEM lysate. Predicted molecular weight: ~47 kDa.