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EIF4G1 (phospho-S1148) polyclonal antibody

Product Specifications

Background

The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4G, eIF4E, and eIF4A. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E binds to the mRNA “cap” during an early step in the initiation of protein synthesis. eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A, and the eIF3 complex .

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Eukaryotic translation initiation factor 4 gamma 1; eIF-4-gamma 1; eIF-4G 1; eIF-4G1; p220; EIF4G1; EIF4F; EIF4G; EIF4GI

Swiss Prot

Q04637

Reactivity

Human

Immunogen

A synthetic peptide corresponding to residues in Human EIF4G1 around the phosphorylation site of S1148.

Conjugation

Unconjugated

Related Pathways

Dopamine Signaling in Parkinson's Disease Plathway

Applications

WB

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylated

Molecular Weight

~ 250 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

EIF4G1 (phospho-S1148) polyclonal antibody detects endogenous levels of EIF4G1 protein around the phosphorylation site of S1148.

Applications Notes

Western blot (WB) analysis of EIF4G1 (phospho-S1148) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (30ug)

Host or Source

Rabbit

CAS Number

9007-83-4

Available Sizes

Curated Selection

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