EIF4G1 (phospho-S1148) polyclonal antibody
Product Specifications
Background
The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4G, eIF4E, and eIF4A. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E binds to the mRNA “cap” during an early step in the initiation of protein synthesis. eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A, and the eIF3 complex .
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Eukaryotic translation initiation factor 4 gamma 1; eIF-4-gamma 1; eIF-4G 1; eIF-4G1; p220; EIF4G1; EIF4F; EIF4G; EIF4GI
Swiss Prot
Q04637
Reactivity
Human
Immunogen
A synthetic peptide corresponding to residues in Human EIF4G1 around the phosphorylation site of S1148.
Conjugation
Unconjugated
Related Pathways
Dopamine Signaling in Parkinson's Disease Plathway
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylated
Molecular Weight
~ 250 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
EIF4G1 (phospho-S1148) polyclonal antibody detects endogenous levels of EIF4G1 protein around the phosphorylation site of S1148.
Applications Notes
Western blot (WB) analysis of EIF4G1 (phospho-S1148) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (30ug)
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
