Napsin A Antibody
Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
O96009
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 50-150) from the human protein was used as the immunogen for the Napsin A antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
NAPSA/7165R
Type
Recombinant
Applications
WB, IHC-P
Purity
Protein A affinity
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Aliquot the Napsin A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Napsin A antibody is available for research use only.
Storage Conditions
Aliquot the Napsin A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the Napsin A antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Western blot testing of human kidney tissue lysate with Napsin A antibody. Expected molecular weight: 38-45 kDa.
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