Anti-ACE2 (human), mAb (blocking) (AC384) Azide free

Angiotensin-converting enzyme2 (ACE2) is an ectoenzyme (carboxypeptidase) with an extracellular catalytic domain that predominantly localizes at the plasma membrane and is thereby able to hydrolyze circulating peptides. ACE2 has approximately 42% sequence identity with ACE and its cytoplasmic and transmembrane domains show 48% homology to the protein collectrin, that plays a critical role in the amino acid absorption of the kidney. ACE2 converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. ACE2 is involved in the regulation of systemic blood pressure and has direct effects on cardiac functions. It is expressed predominantly in endothelial cells of the lung, gut, heart and kidney. ACE2 together with the protease TMPRSS2 acts as a functional receptor for SARS coronavirus as well as for the new highly pathogenic coronavirus, 2019-nCoV/SARS-CoV-2, which is cause for pneumonia COVID-19.

Product Specifications

Product Name Alternative

Anti-ACE2 (human), mAb (blocking) (AC384)

UniProt

Q9BYF1

Reactivity

Human

Immunogen

Recombinant human ACE2.

Clonality

Monoclonal

Clone

AC384

Applications

Functional Assay, ELISA, WB

Purification

Protein G-affinity purified.

Components

1mg/mL in PBS. Sodium Azide free

Storage Conditions

Short Term Storage + 4°C; Long Term Storage -20°C; Handling Advice After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Applications Notes

ELISA: (direct or indirect: 1:2'000-1:10'000) Western blot: (1:2'000-1:10'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Functional Application: Blocks the binding of human ACE2 to the Spike protein of SARS-Cov-2.