Human IL-22/Interleukin-22 ELISA Kit PicoKine®

Interleukin-22 (IL-22), also known as ILTIF, is protein that in humans is encoded by the IL22 gene. IL-22 a member of a group of cytokines called the IL-10 family or IL-10 superfamily a class of potent mediators of cellular inflammatory responses. Using FISH, the IL22 gene is mapped to chromosome 12q15, close to the IFNG and the herpesvirus saimiri-induced AK155 genes. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. In some contexts, the pro-inflammatory versus tissue-protective functions of IL-22 are regulated by the often co-expressed cytokine IL-17A.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 33 38 33 36 3.08 8.5% Sample 2 215 247 244 248 238 13.64 5.7% Sample 3 435 437 429 427 432 4.12 0.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 33 38 33 36 3.08 8.5% Sample 1 40 33 38 33 36 3.08 8.5% Sample 2 215 247 244 248 238 13.64 5.7% Sample 2 215 247 244 248 238 13.64 5.7% Sample 3 435 437 429 427 432 4.12 0.9% Sample 3 435 437 429 427 432 4.12 0.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 215 435 41 220 427 Standard deviation 2.04 14.83 26.97 2.58 18.92 27.32 CV (%) 6.4% 6.9% 6.2% 8.6% 8.6% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 215 435 41 220 427 Standard deviation 2.04 14.83 26.97 2.58 18.92 27.32 CV (%) 6.4% 6.9% 6.2% 8.6% 8.6% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 40 215 435 41 220 427 Mean (pg/ml) 40 215 435 41 220 427 Standard deviation 2.04 14.83 26.97 2.58 18.92 27.32 Standard deviation 2.04 14.83 26.97 2.58 18.92 27.32 CV (%) 6.4% 6.9% 6.2% 8.6% 8.6% 6.4% CV (%) 6.4% 6.9% 6.2% 8.6% 8.6% 6.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.016 0.112 0.226 0.37 0.643 1.114 1.566 1.925 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.016 0.112 0.226 0.37 0.643 1.114 1.566 1.925 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.016 0.112 0.226 0.37 0.643 1.114 1.566 1.925 O.D. 0.016 0.112 0.226 0.37 0.643 1.114 1.566 1.925

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.