Human LPL ELISA Kit PicoKine®
Human LPL PicoKine® ELISA Kit (96 Tests) . Quantitate Human LPL in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 40 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Product Specifications
Background
LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism.
Reproducibility
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 137 100 85 132 114 3.71 3.3% Sample 2 201 296 166 236 225 4.72 2.1% Sample 3 2403 2329 2427 2205 2341 163.17 7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 137 100 85 132 114 3.71 3.3% Sample 1 137 100 85 132 114 3.71 3.3% Sample 2 201 296 166 236 225 4.72 2.1% Sample 2 201 296 166 236 225 4.72 2.1% Sample 3 2403 2329 2427 2205 2341 163.17 7% Sample 3 2403 2329 2427 2205 2341 163.17 7%
Gene Name
LPL
Gene ID
4023
UniProt
P06858
Reactivity
Human
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Immunogen
Expression system for standard: CHO; Immunogen sequence: A28-G475
Capture Antibody
A polyclonal antibody from goat
Detection Antibody
A biotinylated polyclonal antibody from goat
Applications
ELISA
Standard
10 ng/Tube
Sample Type
Cell culture supernatants, serum and plasma (heparin, EDTA)
Detection Range
78 pg/mL - 5,000 pg/mL
Precision
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 137 201 2403 127 163 2010 Standard deviation 9.55 9.27 160.76 5.82 11.8 98.49 CV (%) 7% 4.6% 6.7% 4.6% 7.2% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 137 201 2403 127 163 2010 Standard deviation 9.55 9.27 160.76 5.82 11.8 98.49 CV (%) 7% 4.6% 6.7% 4.6% 7.2% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 137 201 2403 127 163 2010 Mean (pg/ml) 137 201 2403 127 163 2010 Standard deviation 9.55 9.27 160.76 5.82 11.8 98.49 Standard deviation 9.55 9.27 160.76 5.82 11.8 98.49 CV (%) 7% 4.6% 6.7% 4.6% 7.2% 4.9% CV (%) 7% 4.6% 6.7% 4.6% 7.2% 4.9%
Sensitivity
<40 pg/mL
Components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Specificity
Natural and recombinant human LPL
Anticoagulant
Heparin or EDTA
Sample Data
Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.073 0.167 0.225 0.369 0.6259 1.107 1.793 2.4 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.073 0.167 0.225 0.369 0.6259 1.107 1.793 2.4 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.073 0.167 0.225 0.369 0.6259 1.107 1.793 2.4 O.D. 0.073 0.167 0.225 0.369 0.6259 1.107 1.793 2.4
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
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