Human DLL1 ELISA Kit PicoKine®

Human DLL1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human DLL1 in cell culture supernatants, serum, plasma (heparin, EDTA), urine and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

DLL1 (DELTA-LIKE 1), also known as DL1 or DELTA1, is a protein that in humans is encoded by the DLL1 gene. DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis, and it is also important in cell-to-cell communication. This gene is mapped to chromosome 6q27, near a locus for type I diabetes. Functional analysis suggested that a soluble fusion protein containing the DSL domain of DLL1 and its adjacent 50 N-terminal amino acids increased the viability of hemopoietic cells but inhibited cell death. It has been found that DLL1 blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 105 104 94 106 102 4.81 4.7% Sample 2 634 578 595 596 600 20.48 3.4% Sample 3 2353 2202 2459 2342 2339 91.34 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 105 104 94 106 102 4.81 4.7% Sample 1 105 104 94 106 102 4.81 4.7% Sample 2 634 578 595 596 600 20.48 3.4% Sample 2 634 578 595 596 600 20.48 3.4% Sample 3 2353 2202 2459 2342 2339 91.34 3.9% Sample 3 2353 2202 2459 2342 2339 91.34 3.9%

Synonyms

Delta-like protein 1; Drosophila Delta homolog 1; Delta1; H-Delta-1; DLL1; UNQ146/PRO172

Gene Name

DLL1

Gene ID

28514

UniProt

O00548

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S22-G540

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.

Applications

ELISA

Field of Research

Developmental Biology, Embryogenesis, Embryonic Stem Cells, Epigenetics and Nuclear Signaling, Hematopoietic Progenitors, Intracellular, Neurogenesis, Neurology Process, Neuroscience, Signaling Pathways, Stem Cells, Surface Molecules, Transcription

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), urine and human milk.

Detection Range

78 pg/mL - 5,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 105 634 2353 102 662 2503 Standard deviation 4.62 27.26 94.12 4.69 31.11 137.66 CV (%) 4.4% 4.3% 4% 4.6% 4.7% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 105 634 2353 102 662 2503 Standard deviation 4.62 27.26 94.12 4.69 31.11 137.66 CV (%) 4.4% 4.3% 4% 4.6% 4.7% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 105 634 2353 102 662 2503 Mean (pg/ml) 105 634 2353 102 662 2503 Standard deviation 4.62 27.26 94.12 4.69 31.11 137.66 Standard deviation 4.62 27.26 94.12 4.69 31.11 137.66 CV (%) 4.4% 4.3% 4% 4.6% 4.7% 5.5% CV (%) 4.4% 4.3% 4% 4.6% 4.7% 5.5%

Sensitivity

<10 pg/mL

Function

Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

78056 MW

Specificity

Natural and recombinant human DLL1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Delta-like protein 1

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 1 DSL domain.

Protein Name

Delta-like protein 1

Sample Data

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.026 0.113 0.195 0.34 0.6 1.057 1.729 2.199 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.026 0.113 0.195 0.34 0.6 1.057 1.729 2.199 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.026 0.113 0.195 0.34 0.6 1.057 1.729 2.199 O.D. 0.026 0.113 0.195 0.34 0.6 1.057 1.729 2.199

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.