Anti-ch-TOG Antibody

Rabbit Polyclonal Antibody specific to ch-TOG

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Colonic and hepatic tumor overexpressed gene protein, Ch-TOG

Gene Name

CKAP5

NCBI Gene ID

UniProt

Q14008

Cellular Locus

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cytoplasm, cytoskeleton, spindle pole, Cytoplasm, cytoskeleton, spindle, Chromosome, centromere, kinetochore

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

NH2 terminal cysteine-peptide corresponding to aa 2019-2032 in the carboxyl terminal region of human ch- TOG coupled to KLH: NH2-CDLKKRLERIKSSRK-COOH.

Target Antigen

Cytoskeleton-associated protein 5

Target

ch-TOG

Clonality

Polyclonal

Type

Antibody

Applications

WB, IF, IP

Field of Research

Cancer research

Purification Method

Whole antiserum

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Whole Antiserum

Form

Liquid

Buffer

Whole Antiserum

Function

Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Acts as processive microtubule polymerase. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles. In spindle formation protects kinetochore microtubules from depolymerization by KIF2C and has an essential role in centrosomal microtubule assembly independently of KIF2C activity. Contributes to centrosome integrity. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed:23532825). Enhances the strength of NDC80 complex-mediated kinetochore-tip microtubule attachments (PubMed:27156448). {PubMed:12569123, PubMed:18809577, PubMed:21297582, PubMed:21646404, PubMed:23532825, PubMed:27156448, PubMed:9570755}.

Additionnal Information

Immunoblotting: use at 1:5,000-1:10,000 dilution. A band of approx. 215kDa is detected. Immunoprecipitation: use at 1:5,000- 1:10,000 dilution. Immunofluorescence: use at 1:5,000- 1:10,000 dilution. These are recommended concentrations. User should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes human and mouse ch-TOG. A BLAST search demonstrated 100% identity to the peptide immunogen in human, mouse, rat, chimpanzee, dog, cow, chicken, opossum, and frog.

Formulation

Whole antiserum

Target Background

Colonic and hepatic tumor over-expressed protein (ch-TOG) belongs to the TOG/XMAP215/Dis1 evolutionarily ancient family of microtubule-associated proteins with isoforms found in all major kingdoms of eukaryotes. This protein is required for the assembly and function of the meiotic or mitotic spindles during cell division and is localized to microtubule-organizing centers (MTOCs) during interphase or to the spindle poles and centrosomes during mitosis or cytokinesis.