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ME3 rabbit pAb

Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP (+)-dependent isoform, a mitochondrial NADP (+)-dependent isoform, and a mitochondrial NAD (+)-dependent isoform. This gene encodes a mitochondrial NADP (+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008],

Product Specifications

Background

Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP (+) -dependent isoform, a mitochondrial NADP (+) -dependent isoform, and a mitochondrial NAD (+) -dependent isoform. This gene encodes a mitochondrial NADP (+) -dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Synonyms

ME3; NADP-dependent malic enzyme; mitochondrial; NADP-ME; Malic enzyme 3

Gene ID

10873

UniProt

Q16798

Cellular Locus

Mitochondrion matrix.

Host

Rabbit

Species Reactivity

Human,Mouse

Reactivity

Human; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human ME3. AA range:545-594

Clonality

Polyclonal

Isotype

IgG

Source

Rabbit

Applications

WB, IHC, IF, ELISA

Validated Applications

WB,IHC,IF,ELISA

Stability

-20°C/1 year

Concentration

1 mg/mL

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Molecular Weight

67kD

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.elkbiotech.com/upload/file/Antibodies/pAb/ES4565-1.pdf

Observed Molecular Weight

67 kD

Subcellular Location

Mitochondrion matrix.

Other Product Names

ME3; NADP-dependent malic enzyme; mitochondrial; NADP-ME; Malic enzyme 3

Gene ID (Human)

10873

SwissProt (Human)

Q16798

Available Sizes

Curated Selection

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