Recombinant mouse DDT protein
Ddt, also known as D-dopachrome decarboxylase, is an enzyme that catayzes the tautomerization of D-dopachrome to give 5, 6-dihyroxyindole (DHI) . This protein belongs to the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. It shares a homologous amino acid sequence (33% identical) with the macrophage migration inhibitory factor (MIF) and possesses similar tautomerase activity in addition, it may functions a proinflammatory cytokine. Recombinant mouse Ddt, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
D-dopachrome decarboxylase, C78655
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
15.5 kDa (141aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Hiyoshi M., et al. (2009) Toxicology. 255 (1-2) : 6-14.; ; Sugimoto H., et al., (1999) Biochemistry. 38 (11) :3268-79.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_034157
Uniprot Accession Number
O35215
Species
Mouse
AA Sequence
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