Recombinant human SEPT3/SEPTIN3 protein

SEPT3 belongs to the septin family of GTPases. Members of this family are required for cytokinesis. Expression is upregulated by retinoic acid in a human teratocarcinoma cell line. The specific function of this gene has not been determined. Alternative splicing of this gene results in two transcript variants encoding different isoforms. Recombinant human SEPT3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Septin 3, Neuronal-specific septin-3, 41885

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by BRADFORD assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

43.1 kDa (381aa) confirmed by MALDI-TOF

Additionnal Information

SETP 3, SETP-3, Septin-3, Septin 3, Neuronal-specific septin-3, 41885, ATGP2919-10 µg, ATGP2919-20 µg, ATGP2919-50 µg, ATGP2919-100 µg, ATGP2919-250 µg, ATGP2919-500 µg, ATGP2919-1 mg, ATGP2919-010, ATGP2919-020, ATGP2919-050, ATGP2919-100, ATGP2919-250, ATGP2919-500, ATGP2919-01M

References & Citations

Methner A. et al. (2001) Biochem. Biophys. Res. Commun. 283:48-56.; ; Macedo J.N. et al. (2013) Biochem. J. 450:95-105.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol 1mM DTT

Scientific Category

Cell Cycle

NCBI Accession Number

NP_663786.2

Uniprot Accession Number

Q9UH03

Species

Human

AA Sequence

MSKGLPE TRTDAAMSEL VPEPRPKPAV PMKPMSINSN LLGYIGIDTI IEQMRKKTMK TGFDFNIMVV GQSGLGKSTL VNTLFKSQVS RKASSWNREE KIPKTVEIKA IGHVIEEGGV KMKLTVIDTP GFGDQINNEN CWEPIEKYIN EQYEKFLKEE VNIARKKRIP DTRVHCCLYF ISPTGHSLRP LDLEFMKHLS KVVNIIPVIA KADTMTLEEK SEFKQRVRKE LEVNGIEFYP QKEFDEDLED KTENDKIRQE SMPFAVVGSD KEYQVNGKRV LGRKTPWGII EVENLNHCEF ALLRDFVIRT HLQDLKEVTH NIHYETYRAK RLNDNGGLPP GEGLLGTVLP PVPATPCPTA E