Recombinant human TSFM protein

TSFM is a mitochondrial translation elongation factor. It is an enzyme that catalyzes the exchange of guanine nucleotides on the translation elongation factor Tu during the elongation step of mitchondrial protein translation. Mutations in TSFM are associated with combined oxidative phosphorylation deficiency-3 syndrome. Alternate splicing results in multiple transcript variants. Recombinant human TSFM protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Ts translation elongation factor mitochondrial, Ts translation elongation factor, mitochondrial, COXPD3, EF-TS, EF-Tsmt

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

32.9 kDa (302aa)

Additionnal Information

TSFM, Ts translation elongation factor mitochondrial, Ts translation elongation factor, mitochondrial, COXPD3, EF-TS, EF-Tsmt, ATGP2691-10 µg, ATGP2691-20 µg, ATGP2691-50 µg, ATGP2691-100 µg, ATGP2691-250 µg, ATGP2691-500 µg, ATGP2691-1 mg, ATGP2691-10, ATGP2691-20, ATGP2691-50, ATGP2691-100, ATGP2691-250, ATGP2691-500, ATGP2691-1

References & Citations

Xin H. et al. (1995) J Biol Chem. 270:17243-17249.; ; Smeitink JA. et al. (2006) Am. J. Hum. Genet. 79:869-877.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_005717.2

Uniprot Accession Number

P43897

Species

Human

AA Sequence

MSKELLMKLR RKTGYSFVNC KKALETCGGD LKQAEIWLHK EAQKEGWSKA AKLQGRKTKE GLIGLLQEGN TTVLVEVNCE TDFVSRNLKF QLLVQQVALG TMMHCQTLKD QPSAYSKVQW LTPVNLALWE AEAGGSLEGF LNSSELSGLP AGPDREGSLK DQLALAIGKL GENMILKRAA WVKVPSGFYV GSYVHGAMQS PSLHKLVLGK YGALVICETS EQKTNLEDVG RRLGQHVVGM APLSVGSLDD EPGGEAETKM LSQPYLLDPS ITLGQYVQPQ GVSVVDFVRF ECGEGEEAAE TE