BCAM Protein (C-His, Mouse)

Product Specifications

Abbreviation

BCAM

Synonyms

1200005K12Rik, B-CAM, Bcam, Gplu, Lu

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 98 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of HOS human osteogenic sarcoma cells. When cells are added to coated plates (5 μg/mL, 100μL/well), approximately 30-70% will adhere after 1 hour at 37°C.

Function

Met1-Ala541

Molecular Weight

The secreted recombinant mouse BCAM comprises 527 amino acids and has a calculated molecular mass of 58.3 kDa. As a result of glycosylation, the apparent molecular mass of rmBCAM is approximately 70-75 kDa in SDS-PAGE under reducing conditions.