Recombinant human Sirtuin 1/SIRT1 protein

SIRT1 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. Alternative splicing results in multiple transcript variants. Recombinant human SIRT1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

NAD-dependent protein deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1, hSIR2, SIR2L1, Sirtuin, Silent mating type information regulation 2 homolog 1 (S. cerevisiae)

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

31.6 kDa (280aa)

Additionnal Information

Sirtuin1, SIRT1, Sirtuin 1, Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae), SIR2L1, ATGP2625-10 µg, ATGP2625-20 µg, ATGP2625-50 µg, ATGP2625-100 µg, ATGP2625-250 µg, ATGP2625-500 µg, ATGP2625-1 mg, ATGP2625-10, ATGP2625-20, ATGP2625-50, ATGP2625-100, ATGP2625-250, ATGP2625-500, ATGP2625-1

References & Citations

Langley E., et al. (2002) EMBO J. 21:2383-2396.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_036370

Uniprot Accession Number

Q96EB6

Species

Human

AA Sequence

MGRDLYDDDD KDRWGSHMKK IIVLTGAGVS VSCGIPDFRS RDGIYARLAV DFPDLPDPQA MFDIEYFRKD PRPFFKFAKE IYPGQFQPSL CHKFIALSDK EGKLLRNYTQ NIDTLEQVAG IQRIIQCHGS FATASCLICK YKVDCEAVRG DIFNQVVPRC PRCPADEPLA IMKPEIVFFG ENLPEQFHRA MKYDKDEVDL LIVIGSSLKV RPVALIPSSI PHEVPQILIN REPLPHLHFD VELLGDCDVI INELCHRLGG