Recombinant human Cyclin B1 protein
CCNB1, also known as cyclin B1, is a regulatory protein involved in mitosis. This protein complexes with p34 (cdc2) to form the maturation-promoting factor (MPF) . Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. Recombinant human CCNB1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Cyclin B1, CCNB
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
50.9 kDa (457aa)
Additionnal Information
References & Citations
Antinore M J., et al. (1999) . Oncogene 18 (18) :2892-900
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol
Scientific Category
Cell Cycle
NCBI Accession Number
NP_114172.1
Uniprot Accession Number
P14635
Species
Human
AA Sequence
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