Recombinant human ACY3 protein
Aspartoacylase 3, also known as ACY3, is member of the Aspartoacylase subfamily. It plays an important role in deacetylating mercapturic acids in kidney proximal tubules. ACY3 localizes to the cytoplasm of S2 and S3 proximal tubules and to the apical domain of S1 proximal tubules. ACY3 protein is also expressed at low levels in stomach, testis, heart, brain, lung and liver, and may function as an HCV (Hepatitis C virus) core binding protein. Recombinant human ACY3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Aspartoacylase 3, ACY-3, HCBP1, Aminoacylase-3
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
37.6 kDa (342aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Chen Y R., et al. (2009) J Gastroenterol Hepatol. 24 (7) :1300-4.; ; Pushkin A., et al. (2004) . Am J Physiol Cell Physiol. 286 (4) :C848-56.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_542389
Uniprot Accession Number
Q96HD9
Species
Human
AA Sequence
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