Recombinant human TSEN15 protein

TSEN15 (tRNA-splicing endonuclease subunit Sen15) belongs to the SEN15 family. tRNA splicing is a fundamental process required for cell growth and division. SEN15 is a subunit of the tRNA splicing endonuclease. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. Recombinant human TSEN15 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

TRNA-splicing endonuclease subunit Sen15, sen15, C1orf19

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

20.9 kDa (191aa) confirmed by MALDI-TOF

Additionnal Information

TSEN15, tRNA-splicing endonuclease subunit Sen15, sen15, C1orf19, ATGP1072-10 µg, ATGP1072-20 µg, ATGP1072-50 µg, ATGP1072-100 µg, ATGP1072-250 µg, ATGP1072-500 µg, ATGP1072-1 mg, ATGP1072-10, ATGP1072-20, ATGP1072-50, ATGP1072-100, ATGP1072-250, ATGP1072-500, ATGP1072-1

References & Citations

Song J, et al. (2007) J Mol Biol 366 (1) : 155-64.; ; Paushkin, et al. (2004) Cell 117 (3) : 311-21.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200 mM NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_443197

Uniprot Accession Number

Q8WW01

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MEERGDSEPT PGCSGLGPDG VRGFGDGGGA PSWAPEDAWM GTHPKYLEMM ELDIGDATHV YVAFLVYLDL MESKSWHEVN CVGLPELQLI CLVGTEIEGE GLQTVVPTPI TASLSHNRIR EILKASRKLQ GDPDLPMSFT LAIVESDSTI VYYKLTDGFM LPDPQNISLR R