Human HMG-1/HMGB1 antibody

High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor.

Product Specifications

Product Name Alternative

Sulfoglucuronyl carbohydrate binding protein, SBP-1, HMGB1, HMG3, HMG1, High-mobility group box1, High-mobility group (nonhistone chromosomal) protein 1, DKFZp686A04236, Amphoterin

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human HMGB1 (1-215aa) purified from Baculovirus

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

J2E1

Conjugation

Unconjugated

Applications

ELISA, WB, IHC

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Sulfoglucuronyl carbohydrate binding protein, SBP-1, SBP1, SBP 1, HMGB1, HMGB 1, HMG3, HMG1, HMG 1, High-mobility group box1, High-mobility group (nonhistone chromosomal) protein 1, DKFZp686A04236, Amphoterin, HMG 3, High mobility group box1, High mobility group (nonhistone chromosomal) protein 1, High mobility group protein 1, AHM0915-10 µg, AHM0915-20 µg, AHM0915-50 µg, AHM0915-100 µg, AHM0915-250 µg, AHM0915-500 µg, AHM0915-1 mg, AHM0915-010, AHM0915-020, AHM0915-050, AHM0915-100, AHM0915-250, AHM0915-500, AHM0915-01M

References & Citations

Palumbo R J, et al., (2004) Cell Biol. Feb 2 164 (3) .; ; Andersson U, et al., (2002) Leukoc Biol. Dec. 72 (6) :1084-91.

Other References

Hashizume H, et al. Skin recruitment of monomyeloid precursors involves human herpesvirus-6 reactivation in drug allergy. (Allergy. 2013) {https://pubmed.ncbi.nlm.nih.gov/23573902/}; ; Ito H, et al. HMGB1 facilitates repair of mitochondrial DNA damage and extends the lifespan of mutant ataxin-1 knock-in mice. 9EMBO Mol Med. 2015) {https://pubmed.ncbi.nlm.nih.gov/25510912/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_002119

Uniprot Accession Number

P09429

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HMGB1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; Lane 1.: K562 cell lysate; ; Lane 1.: MCF7 cell lysate;

IHC description

Paraffin embedded sections of human colon cancer tissue were incubated with anti-human HMGB1 (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)