CREB (Ser133) Antibody

Product Specifications

Background

It is well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus (Karin and Hunter, 1995) . The cyclic AMP response element binding protein (CREB) is one of the best characterized stimulus-induced transcription factors (Montminy, 1997) . This transcription factor is a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory (Shaywitz and Greenberg, 1999; Silva et al., 1998) . A variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs) phosphorylate CREB at serine 133 (Ser-133), and phosphorylation of Ser-133 are required for CREB-mediated transcription (Johannessen et al., 2004; Kornhauser et al., 2002) .

Synonyms

Active transcription factor CREB antibody, cAMP response element binding protein 1 antibody, cAMP response element binding protein antibody, cAMP responsive element binding protein 1 antibody, cAMP-responsive element-binding protein 1 antibody, CREB antibody, CREB-1 antibody, CREB1 antibody, CREB1_HUMAN antibody, Cyclic AMP-responsive element-binding protein 1 antibody, MGC9284 antibody, OTTHUMP00000163864 antibody, OTTHUMP00000163865 antibody, OTTHUMP00000206660 antibody, OTTHUMP00000206662 antibody, , TTHUMP00000206667 antibody, Transactivator protein antibody

Gene ID

81646

Swiss Prot

P15337

Modification Site

Ser133

Host

Rabbit

Cross Reactivity

Mouse, Rat

Target

CREB (Ser133)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser133 of rat CREB, conjugated to keyhole limpet hemocyanin (KLH) .

Applications

WB, IHC

Purification

Antigen Affinity purification from Pooled whole antiserum

Concentration

Lot Dependent

Dilution

WB (1:300-5000), IHC ()

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Specific for endogenous levels of the ~45 kDa CREB protein phosphorylated at Ser133. Immunolabeling is completely eliminated by treatment with λ-phosphatase.