Zebrafish Ints12 Antibody / Integrator complex subunit 12

Integrator complex subunit 12 (Int12) also known as PHD finger protein 22 (PHF22) is a protein that in humans is encoded by the INTS12 gene. INTS12 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 [PubMed 16239144]) .

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

Q6IQU7

Host

Rabbit

Reactivity

Zebrafish

Immunogen

An E.coli-derived zebrafish Ints12 recombinant protein (amino acids M1-K394) was used as the immunogen for the Zebrafish Ints12 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, IF

Purity

Antigen affinity chromatography

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Limitations

This Zebrafish Ints12 antibody is available for research use only.

Storage Conditions

After reconstitution, the Zebrafish Ints12 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Zebrafish Ints12 antibody should be determined by the researcher.

Location

Nuclear, cytoplasmic, cell membrane

Image Legend

Immunohistochemical analysis of Ints12 protein using Zebrafish Ints12 antibody and paraffin-embedded zebrafish brain tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.