APPBP1 Rabbit Monoclonal Antibody
Product Specifications
Background
Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.
Product Name Alternative
NEDD8 activating enzyme E1 subunit 1; HPP1; ula-1; APPBP1; A-116A10.1
Gene Name
NAE1
Gene ID
8883
Swiss Prot
Q13564
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Conjugation
Unconjugated
Applications
WB, IP
Field of Research
Cell Biology
Purification
Affinity Purification
Dilution
WB 1:500-1:1000, IP 1:20-1:50
Form
Liquid
Buffer
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Modification
Unmodified
Molecular Weight
Calculated MW: 60 kDa; Observed MW: 60 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.
Isotype
IgG
Available Sizes
Curated Selection
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