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Anti-Shank3 Antibody

Our Anti-Shank3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N367/62. It detects mouse and rat Shank3, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Product Specifications

Background

SH3 and multiple ankyrin repeat domains protein 3, or shank 3 (also known as proline-rich synapse-associated protein 2 (ProSAP2) ) is a member of the shank family. Shank 3 binding domains include a SH3 domain, a proline rich domain, a PDZ domain and ankyrin repeat domains. Shank 3 is found in many tissues including heart and spleen, but is most abundantly expressed in brain at the post synaptic density. Shank 3 acts as a scaffold protein anchoring and connecting membrane bound receptors, ion channels and other proteins to the cytoskeleton in neurons to enable cell signaling. In this manner, Shank 3 may play a role in synapse formation and dendritic spine maturation. Mutations in the Shank 3 gene have been associated with neuropsychiatric diseases, including autism spectrm disorder.

Short Description

Our Anti-Shank3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N367/62. It detects mouse and rat Shank3, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, IP, ELISA.

Synonyms

SH3 and multiple ankyrin repeat domains protein 3 (Shank3) (Proline-rich synapse-associated protein 2) (ProSAP2) (SPANK-2)

Gene Name

Shank3 Prosap2

UniProt

Q9JLU4

Host

Mouse

Reactivity

Mouse and Rat

Immunogen

Fusion protein amino acids 538-626 (SH3 domain) of rat Shank3 (accession number Q9JLU4) produced recombinantly in E. Coli

Target

Shank3

Clonality

Monoclonal

Isotype

IgG2a

Clone

N367/62

Type

Primary Antibody

Applications

WB, IHC, ICC, IP, ELISA

Field of Research

Scaffold Proteins

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

190 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

WB Dilution

1:1000

Specificity

Does not cross-react with Shank1 or Shank2

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Rat

WB Brain Quality Control

1

Conjugate

Unconjugated

RRID

AB_2315921

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