TfR Human Recombinant Protein (Biotin)
Recombinant Human Transferrin receptor protein 1 is produced by our Mammalian expression system and the target gene encoding Cys89-Phe760 is expressed with a 6His, Avi tag at N-terminus.
Product Specifications
Product Name Alternative
Transferrin receptor protein 1; TR; TfR; Trfr; T9; p90
UniProt
P02786
Expression Region
Cys89-Phe760
Conjugation
Biotin
Tag
N-6His-Avi
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Bioactivity
ELISA: Immobilized Recombinant Human Transferrin (C-6His) (Cat#orb2994488) at 5μg/ml (100 μl/well) can bind Biotinylated Human TfR (N-6His-Avi) (Cat#orb2992761) . The ED50 of Biotinylated Human TfR (N-6His-Avi) (Cat#orb2992761) is 22.33 ng/ml. (Regularly tested) . BLI: Loaded Anti-Human TFRC mAb (Human IgG1) on Pro-A Biosensor, can bind Biotinylated Human TfR (N-6His-Avi) (Cat#orb2992761) with an affinity constant of 1.69 nM as determined in BLI assay. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 78.1 KDa. Observed: 80-95 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, 5% Mannitol, 0.01% Tween80, pH 7.4.
Available Sizes
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