Human Complement Factor H/CFH ELISA Kit PicoKine®, Carrier-free

Human Complement Factor H/CFH ELISA Kit PicoKine® (96 Tests). Quantitate Human CFH in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alternativecomplement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alternative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation'). This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>3203</td><td>2994</td><td>3170</td><td>3050</td><td>3104</td><td>85.4</td><td>2.7%</td></tr><tr><td>Sample 2</td><td>13518</td><td>12944</td><td>13467</td><td>11714</td><td>12910</td><td>726.54</td><td>5.6%</td></tr><tr><td>Sample 3</td><td>53844</td><td>53833</td><td>53094</td><td>52680</td><td>53362</td><td>497.77</td><td>0.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Complement factor H;H factor 1;CFH;HF, HF1, HF2;

Gene Name

Complement factor H

Gene ID

3075

UniProt

P08603

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Target protein isolated from human plasma

Tissue Specificity

Expressed by the liver and secreted in plasma.

Applications

ELISA

Standard

100 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Detection Range

1.56 ng/ml - 100 ng/ml

Sensitivity

<50 pg/ml

Function

Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b)NBB complex (C5 convertase) in the alternative complement pathway.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)