Human SHMT1 protein
Recombinant human SHMT1 protein is produced by our mammalian expression system and the target gene encoding Met3-Phe483 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
Serine hydroxymethyltransferase cytosolic; SHMT; Glycine hydroxymethyltransferase; Serine methylase
UniProt
P34896
Reactivity
Human
Source
Human cells
Field of Research
Signal Transduction
Endotoxin
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Form
Lyophilized
Storage Conditions
Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Notes
For research use only.
Applications Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Tested Applications
ELISA, MS, SDS-PAGE, WB
Preservative
0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
AA Sequence
MPVNGAHKDA DLWSSHDKML AQPLKDSDVE VYNIIKKESN RQRVGLELIA SENFASRAVL EALGSCLNNK YSEGYPGQRY YGGTEFIDEL ETLCQKRALQ AYKLDPQCWG VNVQPYSGSP ANFAVYTALV EPHGRIMGLD LPDGGHLTHG FMTDKKKISA TSIFFESMPY KVNPDTGYIN YDQLEENARL FHPKLIIAGT SCYSRNLEYA RLRKIADENG AYLMADMAHI SGLVAAGVVP SPFEHCHVVT TTTHKTLRGC RAGMIFYRKG VKSVDPKTGK EILYNLESLI NSAVFPGLQG GPHNHAIAGV AVALKQAMTL EFKVYQHQVV ANCRALSEAL TELGYKIVTG GSDNHLILVD LRSKGTDGGR AEKVLEACSI ACNKNTCPGD RSALRPSGLR LGTPALTSRG LLEKDFQKVA HFIHRGIELT LQIQSDTGVR ATLKEFKERL AGDKYQAAVQ ALREEVESFA SFFPLPGLPD FVDHHHHHH
Entrez
6470
Available Sizes
Frequently Asked Questions
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