PDPN ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Podoplanin
Gene Aliases
AGGRUS; glycoprotein 36; glycoprotein, 36-KD; GP36; Gp38; GP40; HT1A-1; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2) ; OTS8; PA2.26; PA2.26 antigen; podoplanin; T1A; T1A2; T1A-2; T1-alpha; TI1A.
Gene ID
10630
Accession Number
NP_001006625.1
Reactivity
Homo sapiens, Human
Target
Podoplanin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PDPN ELISA Kit (Human) (OKEH01487) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PDPN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PDPN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PDPN captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
Sensitivity
0.067 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
17 kDa
Specificity
Natural and recombinant Human Podoplanin
Protein Length
162
NCBI Gene Symbol
PDPN
Protein Name
Q86YL7
Gene Name URL
PDPN
Nucleotide Accession Number
NM_001006624.1
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog