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HDAC6 Antibody (Phospho-Ser22)

Product Specifications

CAS Number

9007-83-4

Gene Name

Histone deacetylase 6

Gene Aliases

CPBHM; HD6; histone deacetylase 6; JM21; PPP1R90; protein phosphatase 1, regulatory subunit 90; tubulin-lysine deacetylase HDAC6.

Gene ID

10013

Swiss Prot

Q9UBN7

Reactivity

Human|Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human HDAC6 around the phosphorylation site of Ser22.

Target

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) . Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity) . Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

DSTTTRQRRSRQNPQSPPQDSSVTSKRNIKKGAVPRSIPNLAEVKKKGKM

Applications

Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1mg/ml

Reconstitution

-20°C

Molecular Weight

131 kDa

Notes

Formerly GWB-ASB439

Specificity

HDAC6 (Phospho-Ser22) Antibody detects endogenous levels of HDAC6 only when phosphorylated at Ser22.

NCBI Gene Symbol

HDAC6

Host or Source

Rabbit

Protein Name

Histone deacetylase 6

Gene Name URL

HDAC6

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