MED21 antibody - middle region (ARP37735_P050)

Product Specifications

CAS Number

9007-83-4

Gene Name

Mediator complex subunit 21

Gene Aliases

SRB7, SURB7, hSrb7

Gene ID

9412

Swiss Prot

Q13503

Accession Number

NP_004255

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human MED21

Target

MED21 belongs to the Mediator complex subunit 21 family. It is the component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Partner Proteins

MAGEB4; SSC5D; ZNF655; BLOC1S6; MED21; CDK8; MED19; MED26; UBC; HTT; FBXW7; MED11; MED8; MED28; MED29; MED18; MED4; MED16; MED6; MED13; MED12; MED24; MED17; MED14; MED22; MED1; ELAVL1; VAV2; SKP1; THRA; THRB; MED10; TP53; SREBF1; ZC3H13; ZSCAN1; PCBD2; TA

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

DSLPSEESTAALQAASLYKLEEENHEAATCLEDVVYRGDMLLEKIQSALA

Applications

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 100%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

15kDa

Protein Length

144

NCBI Gene Symbol

MED21

Host or Source

Rabbit

Protein Name

Mediator of RNA polymerase II transcription subunit 21

Gene Name URL

MED21

Nucleotide Accession Number

NM_004264

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