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CAND1 Antibody - middle region

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Product Specifications

CAS Number

9007-83-4

Gene Name

Cullin-associated and neddylation-dissociated 1

Gene Aliases

TIP120, TIP120A

Gene ID

55832

Swiss Prot

Q86VP6

Accession Number

NP_060918

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human CAND1

Target

CAND1 enhances transcription from various types of promoters. It is a regulatory protein that interferes with the assembly of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex and thereby down-regulates ubiquitination of target proteins. CAND1 prevents neddylation of CUL1 by physically blocking access to the neddylation site. CAND1 disrupts interactions between CUL1 and SKP1A and between CUL1 and F-box proteins.

Partner Proteins

ISG15; SUMO2; SUMO3; UBE2D1; UBC; UBE2M; CUL1; ASB2; ASB1; PPM1G; PAWR; OSBP; KARS; GARS; CARS; APEH; STAT1; SHMT2; IPO7; SEC23A; JMJD6; HSPA4; TRMT112; ZYX; DCUN1D5; DCUN1D1; SRPK2; UBD; FBXL3; FN1; CFTR; vif; CA9; ECT2; NOTCH1; CUL4A; CUL5; RBX1; ERCC6

Clonality

Polyclonal

Type

Polyclonal Antibody

Sequence

AALLTIPEAEKSPLMSEFQSQISSNPELAAIFESIQKDSSSTNLESMDTS

Applications

WB

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%

Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

136kDa

Protein Length

1230

NCBI Gene Symbol

CAND1

Host or Source

Rabbit

Protein Name

Cullin-associated NEDD8-dissociated protein 1

Gene Name URL

CAND1

Nucleotide Accession Number

NM_018448

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