What is the catalog number for Recombinant Human IL3?

The catalog number for Recombinant Human IL3 is Z100515.

What sizes are available for Recombinant Human IL3?

Recombinant Human IL3 is available in the following size(s): 10 µg.

Is Recombinant Human IL3 in stock?

Recombinant Human IL3 is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant Human IL3

IL3 is produced mainly by T cells following cell activation by antigens and mitogens, but also by keratinocytes, natural killer cells, mast cells, endothelial cells, and monocytes. The analysis of bacterial- derived recombinant IL3 shows that glycosylation is not required for activity. IL3 sequences are evolutionarily less well conserved with human and murine IL3 sharing approximately 29% homology (at the protein level) and murine and rat IL3 sharing approximately 54% homology. IL3 receptors are expressed on macrophages, mast cells, eosinophils, megakaryocytes, basophils, bone marrow progenitor cells and various myeloid leukemia cells. Binding of IL3 to its receptor causes specific phosphorylation of a 150 kDa membrane glycoprotein. Recombinant human IL3 is a non-glycosylated globular protein.

Product Specifications

CAS Number

9000-83-3

Gene Name

IL3

Gene Aliases

IL-3, Mast cell growth factor, MCGF, Multi-CSF, Hematopoietic growth factor, HCGF, P-cell stimulation factor<br/>Recombinant Human Interleukin-3 (IL3)

Gene ID

3562

Accession Number

P08700

Organism

Human

Source

E. coli

Concentration

<1.0 EU/μg of recombinant protein as determined by the LAL method

Purity

>95% as determined by SDS-PAGE

Solubility

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Function

The ED50 as determined by the dose-dependent proliferation of Human TF-1 cells was found less than 0.5 ng/mL

Molecular Weight

15

Shipping Conditions

Ambient Temperature

Storage Conditions

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.