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Recombinant human HYKK protein

HYKK belongs to the aminoglycoside phosphotransferase family. This protein catalyzes the GTP-dependent phosphorylation of 5-hydroxy-L-lysine. Recombinant human HYKK protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

Hydroxylysine kinase, AGPHD1, Aminoglycoside phosphotransferase domain-containing protein 1, LOC123688

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

44.3 kDa (396aa) confirmed by MALDI-TOF

Additionnal Information

HYKK, Hydroxylysine kinase, AGPHD1, Aminoglycoside phosphotransferase domain-containing protein 1, LOC123688, ATGP2854-10 µg, ATGP2854-20 µg, ATGP2854-50 µg, ATGP2854-100 µg, ATGP2854-250 µg, ATGP2854-500 µg, ATGP2854-1 mg, ATGP2854-10, ATGP2854-20, ATGP2854-50, ATGP2854-100, ATGP2854-250, ATGP2854-500, ATGP2854-1

References & Citations

Veiga-da-Cunha, M., et al. (2012) J. Biol. Chem. 287 (10), 7246-7255

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 40% glycerol, 1mM DTT, 0.1mM PMSF

Scientific Category

Other

NCBI Accession Number

NP_001013641

Uniprot Accession Number

A2RU49

Species

Human

AA Sequence

MSSGNYQ QSEALSKPTF SEEQASALVE SVFGLKVSKV RPLPSYDDQN FHVYVSKTKD GPTEYVLKIS NTKASKNPDL IEVQNHIIMF LKAAGFPTAS VCHTKGDNTA SLVSVDSGSE IKSYLVRLLT YLPGRPIAEL PVSPQLLYEI GKLAAKLDKT LQRFHHPKLS SLHRENFIWN LKNVPLLEKY LYALGQNRNR EIVEHVIHLF KEEVMTKLSH FRECINHGDL NDHNILIESS KSASGNAEYQ VSGILDFGDM SYGYYVFEVA ITIMYMMIES KSPIQVGGHV LAGFESITPL TAVEKGALFL LVCSRFCQSL VMAAYSCQLY PENKDYLMVT AKTGWKHLQQ MFDMGQKAVE EIWFETAKSY ESGISM
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