CTLA-4 antibody

ALPS5; CD152; Celiac disease 3 (CELIAC3) ; Cytotoxic T-lymphocyte-associated antigen 4 (CTLA4) ; GRD4; GSE; ICOS; Insulin-dependent Diabetes Mellitus 12 (IDDM12)

Positive Control: Tonsil: A moderate strong membranous and cytoplasmic CTLA4 immunostaining should be seen in a subset of T-lymphocytes with strongest staining in follicular T-helper cells of germinal centres. Negative Control: Tonsil: The majority of lymphocytes in germinal centres and all epithelial cells should stain negative. Cellular Localization: Cell surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.