Human FGF1/FGF acidic Protein

Human FGF1/FGF acidic protein, expressed in E. coli

Product Specifications

Background

FGF1, also known as FGF acidic, ECGF, aFGF and HBGF-1, is a nonglycosylated member of the FGF family of mitogenic peptides. FGF1, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. FGF1 protein shows a wide range of endocrine-like activities. As a multiple function growth factor, this protein is involved in embryo development and tissue repair. Additionally, this protein is considered to function in several important physiological and pathological processes, such as embryonic development, morphogenesis, angiogenesis, wound healing and atheromatosis, carcinogenesis, development, and invasion of cancer.

Product Name Alternative

Fibroblast Growth Factor 1; FGF-1; Acidic Fibroblast Growth Factor; aFGF; Heparin-Binding Growth Factor 1; HBGF-1; Fgf1; Fgf-1; Fgfa

Gene ID

2246

Accession Number

P05230

Tag

FGF1/aFGF

Type

Cytokines and proteins

Applications

No tag

Purity

> 98 % as determined by SDS-PAGE.

Activity

Measured by its ability to induce proliferation in Balb/3T3 cells. The ED50 for this effect is 23.73 ng/mL.

Molecular Weight

15.5 kDa

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Lyophilized Human FGF1/FGF acidic proteinshould be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

Protein Length

The recombinant Human FGF1/FGF acidic protein consists of 140 amino acids and has a predicted molecular mass of 15.5 KD.

Formulation

Lyophilized from sterile 20mM tris, 50mM NaCl, pH 6.5.

Applications Notes

Human

Other Product Names

Fibroblast Growth Factor 1; FGF-1; Acidic Fibroblast Growth Factor; aFGF; Heparin-Binding Growth Factor 1; HBGF-1; Fgf1; Fgf-1; Fgfa

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human FGF1/FGF acidic protein using the buffer we provided not less than 100 µg/mL, which can then be further diluted to other aqueous solutions.