ERK1 Mouse Monoclonal Antibody [7-D6-E5]
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines , and research investigators consider it an important target in the diagnosis and treatment of cancer . Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway.
Product Specifications
Product Name Alternative
Abbreviation
MAP kinase 3,MAPK 3,ERK-1,p44-MAPK
Swiss Prot
P27361 Human
Cellular Locus
Cytoplasm, Nucleus, Membrane, caveola, Cell junction, focal adhesion.
Host
Mouse
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthetic peptide within Human ERK1 aa 301-350 / 379.
Isotype
IgG1
Conjugation
Non-conjugated
Type
Mouse monoclonal Antibody
Applications
WB, IF-Cell, IHC-P
Positive Control
Hela cell lysates, MCF-7, human breast carcinoma tissue, mouse esophagus tissue, mouse stomach tissue.
Concentration
2 mg/mL
Dilution
WB: 1:5,000-1:10,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:3,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 43 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
No
CAS Number
9007-83-4
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