Rat B7-1/CD80 ELISA Kit PicoKine®

The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain.The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 154 156 159 178 161 9.54 5.9% Sample 2 448 401 380 439 417 27.7 6.6% Sample 3 1666 1734 1536 1629 1641 71.48 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 154 156 159 178 161 9.54 5.9% Sample 1 154 156 159 178 161 9.54 5.9% Sample 2 448 401 380 439 417 27.7 6.6% Sample 2 448 401 380 439 417 27.7 6.6% Sample 3 1666 1734 1536 1629 1641 71.48 4.3% Sample 3 1666 1734 1536 1629 1641 71.48 4.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 154 448 1666 165 472 1688 Standard deviation 10.78 21.5 89.96 13.69 29.26 113.09 CV (%) 7% 4.8% 5.4% 8.3% 6.2% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 154 448 1666 165 472 1688 Standard deviation 10.78 21.5 89.96 13.69 29.26 113.09 CV (%) 7% 4.8% 5.4% 8.3% 6.2% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 154 448 1666 165 472 1688 Mean (pg/ml) 154 448 1666 165 472 1688 Standard deviation 10.78 21.5 89.96 13.69 29.26 113.09 Standard deviation 10.78 21.5 89.96 13.69 29.26 113.09 CV (%) 7% 4.8% 5.4% 8.3% 6.2% 6.7% CV (%) 7% 4.8% 5.4% 8.3% 6.2% 6.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.027 0.092 0.162 0.316 0.576 1.062 1.525 2.013 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.027 0.092 0.162 0.316 0.576 1.062 1.525 2.013 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.027 0.092 0.162 0.316 0.576 1.062 1.525 2.013 O.D. 0.027 0.092 0.162 0.316 0.576 1.062 1.525 2.013

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.