AMPK alpha 1 Mouse Monoclonal Antibody [B1-E10]
Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also it acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. It may regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also AMPK phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
MPK subunit alpha-1,ACACA kinase,HMGCR kinase
Swiss Prot
Q13131 Human
Cellular Locus
Cytoplasm, nucleus
Host
Mouse
Species Reactivity
Human
Immunogen
Recombinant protein within Human AMPK alpha 1 aa 321-520 / 559.
Isotype
IgG1
Conjugation
Non-conjugated
Type
Mouse monoclonal Antibody
Applications
WB, IF-Cell
Positive Control
Hela, PANC-1
Concentration
2 mg/mL
Dilution
IF-Cell: 1:100-1:200 ;WB: 1:1,000-1:2,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 64 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
No
Frequently Asked Questions
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