Phospho-NF-kappaB p65 (S529) Recombinant Rabbit Monoclonal Antibody [SP07-00]
Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NκB (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp κB sequence in the immunoglobulin κ light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFκB is activated and NFκB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
RELA
Swiss Prot
Q04206 Human
Cellular Locus
Nucleus, Cytoplasm.
Host
Rabbit
Species Reactivity
Human
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser529 of Human NF-kB p65.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IHC-P, FC, IP
Positive Control
HeLa treated with 100nM Calyculin A and 20ng/mL TNF-α for 15 minutes cell lysate, human lung tissue, human lung squamous cell carcinoma tissue, Daudi.
Concentration
1 mg/mL
Dilution
WB: 1:1,000 ;IHC-P: 1:1,000 ;FC: 1:50 ;IP: Use at an assay dependent concentration.
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 60 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
Frequently Asked Questions
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