ATG14L Rabbit Polyclonal Antibody
Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1. Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK. Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion. Modulates the hepatic lipid metabolism (By similarity).
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
ATG14
Swiss Prot
Q6ZNE5 Human, Q8CDJ3 Mouse, D4A4K3 Rat
Cellular Locus
Endoplasmic reticulum membrane, Cytoplasm, Preautophagosomal structure membrane, autophagosome membrane.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthetic peptide within human ATG14L aa 421-456.
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
WB, IHC-P
Positive Control
Hela cell lysate, MCF-7 cell lysate, HepG2 cell lysate, SiHa cell lysate, rat brain tissue, human colon tissue, mouse testis tissue.
Concentration
1 mg/mL
Dilution
WB: 1:500-1:2,000 ;IHC-P: 1:100-1:500
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 55 kDa.
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No
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