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14-3-3 alpha+beta Recombinant Rabbit Monoclonal Antibody [SD0837]

14-3-3 protein beta/alpha is a protein that in humans is encoded by the YWHAB gene. This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.

Product Specifications

Product Name Alternative

14 3 3 alpha antibody 14 3 3 protein beta/alpha antibody 14-3-3 protein beta/alpha antibody 1433B_HUMAN antibody Brain protein 14 3 3 beta isoform antibody GW128 antibody HS 1 antibody KCIP-1 antibody KCIP1 antibody N-terminally processed antibody Protein 1054 antibody Protein kinase C inhibitor protein 1 antibody YWHAA antibody YWHAB antibody

Abbreviation

KCIP-1, YWHAB

Swiss Prot

P31946 Human, Q9CQV8 Mouse, P35213 Rat

Cellular Locus

Cytoplasm, Melanosome.

Host

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthetic peptide corresponding to C terminal Human 14-3-3 alpha + beta aa 197-246 / 246.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC, IP

Positive Control

Neuro-2a cell lysate, C6 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, Hela cell lysate, HepG2 cell lysate, 293T cell lysate, SK-Br-3 cell lysate, Hela, A431, Neuro-2a , human breast carcinoma tissue, mouse brain tissue, mouse skin tissue, human breast tissue, rat brain tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IF-Cell: 1:100-1:500 ;IHC-P: 1:50-1:200 ;FC: 1:1,000 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 28 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes

CAS Number

9000-83-3

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