Human MCP-1 / CCL2 ELISA Kit PicoKine®

Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Human MCP-1 is a 8.7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 33 37 35 34 2.23 6.5% Sample 2 159 139 156 158 153 8.15 5.3% Sample 3 477 421 423 456 444 23.46 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 33 37 35 34 2.23 6.5% Sample 1 31 33 37 35 34 2.23 6.5% Sample 2 159 139 156 158 153 8.15 5.3% Sample 2 159 139 156 158 153 8.15 5.3% Sample 3 477 421 423 456 444 23.46 5.2% Sample 3 477 421 423 456 444 23.46 5.2%

C-C motif chemokine 2; HC11; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; MCP-1; Monocyte secretory protein JE; Small-inducible cytokine A2; CCL2; MCP1, SCYA2

Angiogenesis, Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Chemokines, Heart Disease, Immunology, Innate Immunity, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes, Response To Hypoxia, Vascular Inflammation

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 159 477 30 148 481 Standard deviation 1.55 8.74 32.91 1.98 9.76 37.51 CV (%) 5% 5.5% 6.9% 6.6% 6.6% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 159 477 30 148 481 Standard deviation 1.55 8.74 32.91 1.98 9.76 37.51 CV (%) 5% 5.5% 6.9% 6.6% 6.6% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 31 159 477 30 148 481 Mean (pg/ml) 31 159 477 30 148 481 Standard deviation 1.55 8.74 32.91 1.98 9.76 37.51 Standard deviation 1.55 8.74 32.91 1.98 9.76 37.51 CV (%) 5% 5.5% 6.9% 6.6% 6.6% 7.8% CV (%) 5% 5.5% 6.9% 6.6% 6.6% 7.8%

Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.015 0.075 0.126 0.25 0.459 0.875 1.463 2.275 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.015 0.075 0.126 0.25 0.459 0.875 1.463 2.275 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.015 0.075 0.126 0.25 0.459 0.875 1.463 2.275 O.D. 0.015 0.075 0.126 0.25 0.459 0.875 1.463 2.275

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.