MEK1/2 Recombinant Rabbit Monoclonal Antibody [SR13-07]
A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.
Product Specifications
Product Name Alternative
Abbreviation
MAP kinase kinase 1, MAPKK 1, MKK1, MEK 1, MAP kinase kinase 2, MAPKK 2, MEK 2
Swiss Prot
P36507 Human, Q02750 Human, P31938 Mouse, Q63932 Mouse, P36506 Rat, Q01986 Rat
Cellular Locus
Cytoplasm, Nucleus, Membrane, Mitochondrion
Host
Rabbit
Species Reactivity
Human,Mouse,Rat,Zebrafish
Immunogen
Recombinant protein within Human MEK1 aa 1-393 / 393.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
Positive Control
Concentration
1 mg/mL
Dilution
WB: 1:5,000 ;IF-Cell: 1:50 ;IF-Tissue: 1:50 ;IHC-P: 1:200 ;IP: Use at an assay dependent concentration. ;FC: 1:1,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 44 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
CAS Number
9000-83-3
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